WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.

Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

HeLa nuclear fraction and cytoplasmic fraction were subjected to SDS PAGE saparately, followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped, reblotted with lamin B1 (12987-1-AP) & GAPDH (60004-1-Ig) antibodies as the loading control.

IP result of anti-NF-κB p65 (IP:10745-1-AP, 3ug; Detection:10745-1-AP 1:1000) with HeLa cells lysate 5000ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10745-1-AP (p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were stained with 0.2ug p65; RELA antibody (10745-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 NF-κB p65 antibody (CL594-10745) at dilution of 1:100, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green). DAPI (Blue).

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®488 NF-κB p65 antibody (CL488-10745, green) at dilution of 1:100, CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). DAPI (blue).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human NF-κB p65 (CL647-10745) (red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

p65; RELA fusion protein Ag1199 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 80979-1-RR (NF-κB p65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

WB result of NF-κB p65 antibody (80979-1-RR; 1:13000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NF-κB p65 transfected HEK-293 cells.

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using p65; RELA antibody (80979-1-RR, Clone: 4C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NF-κB p65 (80979-1-RR, Clone:4C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Various lysates were subjected to SDS PAGE followed by western blot with 80979-1-RR (NF-κB p65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

WB result of NF-κB p65 antibody (80979-1-RR; 1:13000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NF-κB p65 transfected HEK-293 cells. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using p65; RELA antibody (80979-1-RR, Clone: 4C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NF-κB p65 (80979-1-RR, Clone:4C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

WB result of p65; RELA antibody (66535-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p65; RELA transfected HEK-293 cells.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66535-1-Ig (p65; RELA antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 66535-1-Ig (NF-κB p65 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NF-κB p65 antibody (66535-1-Ig, Clone: 1B12D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).